Publications
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Book
Phage Display in Biotechnology and Drug Discovery
Edited by Sachdev S. Sidhu, CRC Press, New York.
A seventeen chapter volume that details the theories, principles, and methods impacting the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies.
Until recently, the only source for information on the impact of phage display technology on drug discovery research has been laboratory manuals. A thorough reference guide and textbook, Phage Display in Biotechnology and Drug Discovery changes that scenario. It presents the theory and methods behind phage display technology, applications for peptide phage display, protein phage display, and the crucial role of phage display in the development of antibodies.
Highlighting the role that phage display has and will continue to play in the discovery and development of protein therapeutics, this book provides a comprehensive overview that will excite anyone interested in recombinant antibodies.
Year 2012
Two synthetic antibodies that recognize and neutralize distinct proteolytic forms of the Ebola virus envelope blycoprotein.
Koellhoffer, J. F., Chen, G., Sandesara, R. G., Bale, S., Ollmann Saphire, E., Chandran, K., Sidhu, S. S. & Lai, J. R.
ACS Chem. Biol. [Oct 30; Epub ahead of print]. (2012)
A short DNA aptamer that recognizes TNF-alpha and blocks its activity in vitro.
Orava, E. W., Jarvik, N., Shek, Y. L., Sidhu, S. S. & Gariepy, J.
ACS Chem. Biol. [Oct 24; Epub ahead of print]. (2012)
Superbinder SH2 Domains Act as Antagonists of Cell Signaling.
Kaneko, T., Huang, H., Cao, X., Li, X., Li, C., Voss, C., Sidhu, S. S. & Li, S. S.
Sci. Signal. 5, ra68. (2012)
Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography.
Mandal, K., Uppalapati, M., Ault-Riché, D., Kenney, J., Lowitz, J., Sidhu, S. S. & Kent, S. B.
Proc. Natl. Acad. Sci. USA 109, 14779-84. (2012)
T cell receptor-like recognition of tumor in vivo by synthetic antibody fragment.
Miller, K. R., Koide, A., Leung, B., Fitzsimmons, J., Yoder, B., Yuan, H., Jay, M., Sidhu, S. S., Koide, S. & Collins, E. J.
PLoS One 7, e43746. (2012)
Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains.
Tonikian, R., & Sidhu, S. S.
Methods Mol. Biol. 911, 327-53. (2012)
Conformational Control of the Ste5 Scaffold Protein Insulates Against MAP Kinase Misactivation.
Zalatan, J. G., Coyle, S. M., Rajan, S., Sidhu, S. S. & Lim, W. A.
Science 337, 1218-22. (2012)
Synthetic antibodies - concepts, practical considerations and potential.
Miersch, S. & Sidhu, S. S.
Methods 57, 486-98. (2012)
Synthetic antibody libraries.
Nelson, B. & Sidhu, S. S.
Methods Mol. Biol. 899, 27-41. (2012)
Recombinant antibodies and in vitro selection technologies.
Geyer, C. R., McCafferty, J., Dübel, S., Bradbury, A. R. & Sidhu, S. S.
Methods Mol. Biol. 901, 11-32. (2012)
Elucidation of the binding preferences of peptide recognition modules: SH3 and PDZ domains.
Teyra, J., Sidhu, S. S. & Kim, P. M.
FEBS Lett. 586, 2631-7. (2012)
Antibodies for All: The Case for Genome-wide Affinity Reagents.
Sidhu, S. S.
FEBS Lett. 586, 2778-9. (2012)
Synthetic human monoclonal antibodies towards staphylococcal enterotoxin B (SEB) protective against toxic shock syndrome.
Karauzum, H.*, Chen, G.*, Abaandou, L., Mahmoudieh, M.,
Boroun, A. R., Shulenin, S., Devi, V. S., Stavale, E.,
Warfield, K. L., Zeitlin, L., Roy, C. J., Sidhu, S. S.
& Aman, M. J.
* equal contribution
J. Biol. Chem. 287, 25203-15. (2012)
Synthetic antibodies as tools to probe RNA-binding protein function.
Laver, J. D., Ancevicius, K., Sollazzo, P., Westwood, J. T., Sidhu, S. S., Lipshitz, H. D. & Smibert, C. A.
Molec. Biosyst. 8, 1650-7. (2012)
Deciphering the molecular bases of the biological effects of antibodies against Interleukin-2: a versatile platform for fine epitope mapping.
Rojas, G., Pupo, A., Leon, K., Avellanet, J., Carmenate, T. & Sidhu, S. S.
Immunobiol., [in press]
MUSI: an integrated system for identifying multiple specificity from very large peptide or nucleic acid data sets.
Kim, T., Tyndel, M. S., Huang, H., Sidhu, S. S., Bader, G. D., Gfeller, D. & Kim, P. M.
Nucl. Acids Res. 40, e47. (2012)
Charged and hydrophobic surfaces on the a chain of shiga-like toxin 1 recognize the C-terminal domain of ribosomal stalk proteins.
McCluskey, A. J., Bolewska-Pedyczak, E., Jarvik, N., Chen, G., Sidhu, S. S. & Gariépy, J.
PLoS One 7, e31191. (2012)
Simplified synthetic antibody libraries.
Rajan, S. & Sidhu, S. S.
Methods Enzymol. 502, 3-23. (2012)
Year 2011
Studying binding specificities of peptide recognition modules by high-throughput phage display selections.
Huang, H. & Sidhu, S. S.
Methods Mol. Biol. 781, 87-97. (2011)
Identification of specificity determining residues in peptide recognition domains using an information theoretic approach applied to large-scale binding maps.
Yip, K.Y., Utz, L., Sitwell, S., Hu, X., Sidhu, S. S., Turk, B. E., Gerstein, M. B. & Kim, P. M.
BMC Biol. 9, 53. (2011)
Phage display of tissue inhibitor of metalloproteinases-2 (TIMP-2): Identification of selective inhibitors of collagenase-1 (MMP-1).
Bahudhanapati, H., Zhang, Y., Sidhu, S. S. & Brew, K.
Biol. Chem. 286, 31761-70. (2011)
A roadmap to generate renewable protein binders to the human proteome.
Colwill, K.; Renewable Protein Binder Working Group; In vitro Antibody Consortium, Persson, H., Jarvik, N. E., Wyrzucki, A., Wojcik, J., Koide, A., Kossiakoff, A. A., Koide, S., Sidhu, S. S., Dyson, M. R., Pershad, K., Pavlovic, J. D., Karatt-Vellatt, A., Schofield, D. J., Kay, B. K., McCafferty, J., Mersmann, M., Meier, D., Mersmann, J., Helmsing, S., Hust, M., Dübel, S.; Monash Antibody Technologies Facility, Berkowicz, S., Freemantle, A., Spiegel, M., Sawyer, A., Layton, D., Nice, E.; Pawson Laboratory, Dai, A., Rocks, O., Williton, K., Fellouse, F. A., Hersi, K., Pawson, T.; Human Protein Atlas, Nilsson, P., Sundberg, M., Sjöberg, R., Sivertsson, A., Schwenk, J. M., Takanen, J. O., Hober, S., Uhlén, M.; Structural Genomics Consortium, Dahlgren, L. G., Flores, A., Johansson, I., Weigelt, J., Crombet, L., Loppnau, P., Kozieradzki, I., Cossar, D., Arrowsmith, C. H., Edwards, A. M., Gräslund, S.
Nat. Methods 8, 551-8. (2011)
The multiple-specificity landscape of modular peptide recognition domains.
Gfeller, D., Butty, F., Wierzbicka, M., Verschueren, E., Vanhee, P., Huang, H., Ernst, A., Dar, N., Stagljar, I., Serrano, L., Sidhu, S. S., Bader, G. D. & Kim, P. M.
Mol. Syst. Biol. 7, 484. (2011)
Development of reagents and assays for the detection of pathogenic Burkholderia species.
Qazi, O., Rani, M., Gnanam, A. J., Cullen, T. W., Stead, C. M., Kensing, H., McCaul, K., Ngugi, S., Prior, J. L., Lipka, A., Nagy, J. M., Gregory, C. W., Judy, B. M., Harding, S. V., Titball, R. W., Sidhu, S. S., Trent, M. S., Kitto, G. B., Torres, A., Estes, D. M., Iverson, B., Georgiou, G. & Brown, K. A.
Faraday Discuss. 149, 23-36. (2011)
Beyond natural antibodies: the power of in vitro display technologies.
Bradbury, A. R., Sidhu, S. S., Dübel, S., & McCafferty, J.
Nat. Biotechnol. 29, 245-54. (2011)
Evolving specificity from variability for protein interaction domains.
Kaneko, T., Sidhu, S. S. & Li, S. S.
Trends Biochem. Sci. 36, 183-90. (2011)
A portable RNA sequence whose recognition by a synthetic antibody facilitates structural determination.
Koldobskaya, Y., Duguid, E. M., Shechner, D. M., Suslov, N. B., Ye, J., Sidhu, S. S., Bartel, D. P., Koide, S., Kossiakoff, A. A. & Piccirilli, J. A.
Nat. Struct. Mol. Biol. 18, 100-6. (2011)
Year 2010
Functional complex between YAP2 and ZO-2 is PDZ domain dependent, regulates YAP2 nuclear localization and signaling.
Oka, T., Remue, E., Meerschaert, K., Vanloo, B., Boucherie, C., Gfeller, D., Bader, G. D., Sidhu, S. S., Vandekerckhove, J., Gettemans, J. & Sudol, M.
Biochem. J. 432, 461-72. (2010)
Coevolution of PDZ domain-ligand interactions analyzed by high-throughput phage display and deep sequencing.
Ernst, A., Gfeller, D., Kan, Z., Seshagiri, S., Kim, P. M., Bader, G. D. & Sidhu, S. S.
Mol. Biosyst. 6, 1782-90. (2010)
Structure of the complex between HER2 and an antibody paratope formed by side chains from tryptophan and serine.
Fisher, R. D., Ultsch, M., Lingel, A., Schaefer, G., Shao, L., Birtalan, S., Sidhu, S. S. & Eigenbrot, C.
J. Mol. Biol. 402, 217-29. (2010)
MOTIPS: Automated motif analysis for predicting targets of modular protein domains.
Lam, H. Y. K., Kim, P. M., Mok, J., Tonikian, R., Sidhu, S. S., Turk, B. E., Snyder, M. & Gerstein, M. B.
BMC Bioinformatics 11, 243. (2010)
The functional capacity of the natural amino acids for molecular recognition.
Birtalan, S., Fisher, R. D. & Sidhu, S. S.
Mol. Biosyst. 6, 1186-94. (2010)
Year 2009
Mapping protein function by combinatorial mutagenesis.
Pal, G. & Sidhu, S. S.
In "Computational protein-protein interactions" (R. Nussinov & G. Schreiber, Eds), pp 57-86, CRC Press: Boca Raton, FL. (2009)
Phage display.
Ernst, A. & Sidhu, S. S.
In "Protein Engineering and Design" (S. Park and J. Cochran, Eds), pp 1-22, Taylor and Francis: Boca Raton. (2009)
Improving therapeutic efficacy of a complement receptor by structure-based affinity maturation.
Li, B., Xi, H., Diehl, L., Lee, W. P., Sturgeon, L., Chinn, J., DeForge, L., Kelley, R. F., Wiesmann, C., van Lookeren Campagne, M. & Sidhu, S. S.
J. Biol. Chem. 284, 35605-11. (2009)
Bayesian modeling of the yeast SH3 domain interactome predicts spatiotemporal dynamics of endocytosis proteins.
Tonikian, R., Xin, X., Toret, C. P., Gfeller, D., Landgraf, C., Panni, S., Paoluzi, S., Castagnoli, L, Currell, B., Seshagiri, S., Yu, H. Winsor, B., Vidal, M., Davidson, A. R., Gerstein, M. B., Bader, G. D., Volkmer, R., Cesarini, G., Drubin, D. G., Kim, P. M., Sidhu, S. S. & Boone, C.
PLoS Biol. 7, e1000218. (2009)
Rapid evolution of functional complexity in a domain family.
Ernst, A., Sazinsky, S. L., Hui, S., Currell, B., Dharsee, M., Seshagiri, S., Bader, G. D. & Sidhu, S. S.
Sci. Signal. 2, ra50. (2009)
Crystal structure of full-length KcsA in its closed conformation.
Uysal, S., Vasquez, V., Esaiki, K.,Fellouse, F. A., Sidhu, S. S., Koide, S., Perozo, E. & Kossiakoff, A. A.
Proc. Natl. Acad. Sci. USA 106, 6644-9. (2009)
The importance of being tyrosine: Lessons in molecular recognition from minimalist synthetic binding proteins.
Koide, S. & Sidhu, S. S.
ACS Chem. Biol. 4, 325-34. (2009)
Inhibition of Wnt/β-catenin signaling by peptides that target dishevelled PDZ domains.
Zhang, Y., Appleton, B. A., Wiesmann, C., Lau, T., Costa, M., Hannoush, R. N. & Sidhu, S. S.
Nat. Chem. Biol. 5, 217-9. (2009)
Two-state selection of conformation-specific antibodies.
Gao, J., Sidhu, S. S. & Wells, J. A.
Proc. Natl. Acad. Sci. USA 106, 3071-6. (2009)
Year 2008
A specificity map for the PDZ domain family.
Tonikian, R., Zhang, Y., Sazinsky, S. L., Currell, B., Yeh, J.-H., Reva, B., Held, H. A., Appleton, B. A., Evangelista, M., Wu, Y., Xin, X., Chan, A. C., Seshagiri, S., Sander, C., Lasky, L. A., Boone, C., Bader, G. D. & Sidhu, S. S.
PLoS Biol. 6, 2043-59. (2008)
Ubiquitin chain editing revealed by polyubiquitin linkage-specific antibodies.
Newton, K., Matsumoto, M. L., Wertz, I. E., Kirkpatrick, D. S., Lill, J. R., Tan, J., Dugger, D., Gordon, N., Sidhu, S. S., Fellouse, F. A., Komuves, L., French, D. M., Ferrando, R. E., Lam, C., Compaan, D., Yu, C., Bosanac, I., Hymowitz, S. G., Kelly, R. F. & Dixit, V. M.
Cell 134, 668-78. (2008)
A dominant conformational role for amino acid diversity in minimalist protein-protein interfaces.
Gilbreth, R. N., Esaki, K., Koide, A., Sidhu, S. S. & Koide, S.
J. Mol. Biol. 381, 407-18. (2008)
Submitting antibodies to binding arbitration.
Michnick, S. W. & Sidhu, S. S.
Nat. Chem. Biol. 4, 326-29. (2008)
Regulation of a late phase of T cell polarity and effector functions by Crtam.
Yeh, J.-H., Sidhu, S. S. & Chan, A. C.
Cell 132, 846-59. (2008)
The intrinsic contributions of tyrosine, serine, glycine and arginine to the affinity and specificity of antibodies.
Birtalan, S., Zhang, Y., Fellouse, F. A., Shao, L., Schaefer, G. & Sidhu, S. S.
J. Mol. Biol. 337, 1518-28. (2008)
Comprehensive analysis of the factors contributing to the stability and solubility of autonomous human VH domains.
Barthelemy, P. A., Raab, H., Appleton, B. A., Bond, C. J., Wu, P., Wiesmann, C. & Sidhu, S. S.
J. Biol. Chem. 138, 3639-54. (2008)
Synthetic antibodies for specific recognition and crystallization of structured RNA.
Ye, J.-D., Tereshko, V., Frederiksen, J. K., Koide, A., Fellouse, F. A., Sidhu, S. S., Koide, S., Kossiakoff, A. A. & Piccirilli, J. A.
Proc. Natl. Acad. Sci. USA. 105, 82-7. (2007)
Year 2007
The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways.
Alto, N. M., Weflen, A. W., Rardin, M. J., Yarar, D., Lazar, C. S., Tonikian, R., Koller, A., Taylor, S. S., Boone, C., Sidhu, S. S., Schmid, S. L., Hecht, G. A. & Dixon, J. E.
1J. Cell Biol. 78, 1265-78. (2007)
Phage display for engineering and analyzing protein interaction interfaces.
Sidhu, S. S. & Koide, S.
Curr. Opin. Struct. Biol. 17, 481-7. (2007)
High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Fellouse, F. A., Esaki, K., Birtalan, S., Raptis, J., Cancasci, V. J., Koide, A., Jhurani, P., Vasser, M., Wiesmann, C., Kossiakoff, A. A., Koide, S. & Sidhu, S. S.
J. Mol. Biol. 373, 924-40. (2007)
Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3.
Runyon, S. T., Zhang, Y., Appleton, B. A., Sazinsky, S. L., Wu, P., Pan, B., Wiesmann, C., Skelton, N. J. & Sidhu, S. S.
Protein Sci. 16, 2454-71. (2007)
Structural and functional analysis of the ligand specificity of the HtrA2/Omi PDZ domain.
Zhang, Y., Appleton, B. A., Wu, P., Wiesmann, C. & Sidhu, S. S.
Protein Sci. 16, 1738-50. (2007)
Identifying specificity profiles for peptide binding modules with phage-displayed peptide libraries.
Tonikian, R., Zhang, Y., Boone, C. & Sidhu, S. S.
Nat. Protoc. 2, 1368-86. (2007)
Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast.
Gupta, R., Kus, B., Fladd, C., Wasmuth, J., Tonikian, R., Sidhu, S. S., Krogan, N. J., Parkinson, J. & Rotin, D.
Mol. Syst. Biol. 3, 116. (2007)
Exploring and designing protein function with restricted diversity.
Sidhu, S. S. & Kossiakoff, A. A.
Curr. Opin. Chem. Biol. 11, 347-54. (2007)
Full-length antibodies on display.
Sidhu, S. S.
Nat. Biotechnol. 25, 537-8. (2007)
Making antibodies in bacteria.
Fellouse, F. A. & Sidhu, S. S.
In "Making and using antibodies" (G. C. Howard & M. R. Kaser, Eds.), pp 157-180, CRC Press, Boca Raton, FL. (2007)
Year 2006
Synthetic therapeutic antibodies.
Sidhu, S. S. & Fellouse, F. A.
Nat. Chem. Biol. 12, 682-88. (2006)
Engineering the M13 bacteriophage coat protein for improved phage display.
Sidhu, S. S., Feld, B. K. & Weiss, G. A.
In "Methods in molecular biology, volume 352: Protein engineering protocols" (K. M. Arndt and K. M. Muller, Eds.), pp 205-19, Humana Press Inc., Totowa, NJ. (2006)
Activation of the proapoptotic death receptor DR5 by oligomeric peptide and antibody agonists.
Li, B., Totpal, K., Russell, S., Yeh, S., Wong, W. L., Cochran, A., Ashkenazi, A., Hymowitz, S & Sidhu, S. S.
J. Mol. Biol. 361, 522-36. (2006)
Comprehensive and Quantitative mapping of energy landscapes for protein-protein interactions by rapid combinatorial scanning.
Pal, G., Kouadio, J.-L., Artis, D. R., Kossiakoff, A. A. & Sidhu, S. S.
J. Biol. Chem. 281, 22378-85. (2006)
JBC paper of the week. Cover feature in ASBMB Today (October, 2006).
Comparative structural analysis of the Erbin PDZ domain and the first PDZ domain of ZO-1: Insights into determinants of PDZ domain specificity.
Appleton, B. A., Zhang, Y., Wu, P., Yin, J. P., Hunziker, W., Skelton, N. J. Sidhu, S. S. & Wiesmann, C.
J. Biol. Chem. 281, 22312-20. (2006)
Convergent and divergent ligand specificity amongst the PDZ domains of the LAP and zonula occludens (ZO) families.
Zhang, Y., Yeh, S., Appleton, B. A., Held, H. A., Kausalya, J. P., Phua , D. C. Y., Wong, W. L., Lasky, L. A., Wiesmann, C., Hunziker, W. & Sidhu, S. S.
J. Biol. Chem. 281, 22299-311. (2006)
Tyrosine plays a dominant functional role in the paratope of a synthetic antibody derived from a four amino acid code.
Fellouse, F. A., Barthelemy, P. A., Kelley, R. F. & Sidhu, S. S.
J. Mol. Biol. 357, 100-14. (2006)
Synthetic antibody libraries.
Fellouse, F. A. & Sidhu, S. S.
In "Phage display in drug discovery and Biotechnology" (S. S. Sidhu, Ed.), pp 709-740. CRC Press, Boca Raton, FL. (2006)
Year 2005
Alternative views of functional protein binding epitopes obtained by combinatorial shotgun scanning mutagenesis.
Pal, G., Fong, S.-Y., Kossiakoff, A. A. & Sidhu, S. S.
Protein Sci. 14, 2405-13. (2005)
Molecular recognition by a binary code.
Fellouse, F. A., Peden, A. A. & Sidhu, S. S.
J. Mol. Biol. 348, 1153-62. (2005)
Cover feature.
A structure-based database of antibody variable domain diversity.
Bond, C., Wiesmann, C., Marsters, J. C. & Sidhu, S. S.
J. Mol. Biol. 348, 699-709. (2005)
Intramolecular cooperativity in a protein binding site assessed by combinatorial shotgun scanning mutagenesis
Pal, G., Ultsch, M. H., Clark, P. C., Currell, B., Kossiakoff, A. A. & Sidhu, S. S.
J. Mol. Biol. 347, 489-94. (2005)
PDZ domains: Intracellular mediators of carboxy-terminal protein recognition and scaffolding.
Lasky, L. A., Skelton, N. J. & Sidhu, S. S.
In "Modular protein domains" (G. Cesarini, M. Gimona, M. Sudol & M. Yaffe, Eds.), pp 257-278, Wiley-VCH Inc., Germany. (2005)
Year 2004
Synthetic antibodies from a four amino acid code: A dominant role for tyrosine in antigen recognition.
Fellouse, F. A., Wiesmann, C. & Sidhu, S. S.
Proc. Natl. Acad. Sci. USA 101, 12467-72. (2004)
High affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold.
Lee, C. V., Liang, W.-C., Dennis, M. S., Eigenbrot, C., Sidhu, S. S. & Fuh, G.
J. Mol. Biol. 340, 1073-93. (2004)
Phage display-derived peptide-binding provides structural insight into insulin-like growth factor I function.
Skelton, N. J., Schaffer, M. L., Deshayes, K., Blandl, T., Runyon, S., Nakamura, G. & Sidhu, S. S.
Spectroscopy 18, 237-49. (2004)
Comprehensive mutational analysis of the M13 major coat protein: Improved scaffolds for C-terminal phage display.
Held, H. A. & Sidhu, S. S.
J. Mol. Biol. 340, 587-97. (2004)
Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions.
Sidhu, S. S., Li, B., Chen, Y., Fellouse, F. A., Eigenbrot, C. & Fuh, G.
J. Mol. Biol. 338, 299-310. (2004)
Bivalent antibody phage display mimics natural immunoglobulin.
Lee, C. V., Sidhu, S. S. & Fuh, G.
J. Immunol. Methods 284, 119-32. (2004)
Definition of the consensus motif recognized by gamma-adaptin ear domains.
Mattera, R., Ritter, B., Sidhu, S. S., McPherson, P. S. & Bonifacino, J. S.
J. Biol. Chem. 279, 8018-28. (2004)
Year 2003
Comprehensive mutagenesis of the C-terminal domain of the M13 gene-3 minor coat protein: The requirements for assembly into the bacteriophage particle.
Weiss, G. A., Roth, T. A., Baldi, P. F. & Sidhu, S. S.
J. Mol. Biol. 332, 777-82. (2003)
Complex with a phage display-derived peptide provides insight into the function of insulin-like growth factor I.
Schaffer, M. L., Deshayes, K., Nakamura, G. R., Sidhu, S. S. & Skelton, N. J.
Biochem. 42, 9324-34. (2003)
Contributions of CDR3 to VHH domain stability and the design of monobody scaffolds for naïve antibody libraries.
Bond, C., Marsters, J. C. Jr. & Sidhu, S. S.
J. Mol. Biol. 332, 643-55. (2003)
The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: Insights into the mechanisms responsible for improved affinity.
Pal, G., Kossiakoff, A. A., Sidhu, S. S.
J. Mol. Biol. 332, 195-204. (2003)
Constructing phage display libraries by oligonucleotide-directed mutagenesis.
Sidhu, S. S. & Weiss, G. A.
In "Phage display: A practical approach" (T. Clackson and H. B. Lowman, Eds.), pp 27-41, Oxford University Press. (2003)
Origins of PDZ domain ligand specificity: Structure determination and mutagenesis of the Erbin PDZ domain.
Skelton, N. J., Koehler, M. F. T., Zobel, K., Wong, W. L., Yeh, S., Pisabarro, M. T., Yin, J. P., Lasky, L. A. & Sidhu, S. S.
J. Biol. Chem. 278, 7645-54. (2003)
Functional genomics of intracellular peptide recognition domains with combinatorial biology methods.
Sidhu, S. S., Bader, G. D. & Boone, C.
Curr. Opin. Chem. Biol. 7, 97-102. (2003)
Exploring protein-protein interactions with phage display.
Sidhu, S. S., Fairbrother, W. J. & Deshayes, K.
Chembiochem. 4, 14-25. (2003)
Cover feature.
Year 2002
DNA-encoded peptide libraries and drug discovery.
Sidhu, S. S. & Weiss, G. A.
In "Anticancer drug development" (B. Baguley and D. Kerr, Eds.), pp 237-48, Academic Press, San Diego. (2002)
A minimized M13 coat protein defines the requirements for assembly into the bacteriophage particle.
Roth, T. A., Weiss, G. A., Eigenbrot, C. & Sidhu, S. S.
J. Mol. Biol. 322, 357-67. (2002)
The Erbin PDZ domain binds with high affinity and specificity to the carboxyl termini of δ-catenin and ARVCF.
Laura, R. P., Witt, A. S., Held, H. A., Gerstner, R., Deshayes, K., Koehler, M. F. T., Kosik, K. S., Sidhu, S. S. & Lasky, L. A.
J. Biol. Chem. 277, 12906-14. (2002)
Comprehensive functional maps of the antigen-binding site of an anti-ErbB2 antibody obtained with shotgun scanning mutagenesis.
Vajdos, F. F., Adams, C. W., Breece, T. N., Presta, L. G., de Vos, A. M. & Sidhu, S. S.
J. Mol. Biol. 320, 415-28. (2002)
Rapid identification of small binding motifs with high-throughput phage display: Discovery of peptidic antagonists of IGF-1.
Deshayes, K., Schaffer, M., Skelton, N. J., Nakamura, G., Kadkhodayan, S. & Sidhu, S. S.
Chem. Biol. 9, 495-505. (2002)
Year 2001
Getting what you want from phage display.
Sidhu, S. S.
Trends Microbiol. 9, 565. (2001)
Phage display: Increasing the rewards from genomic information.
Sidhu, S. S.
Drug Discov. Today 6, 936. (2001)
Mapping protein-protein interactions with combinatorial biology methods.
Pelletier, J. & Sidhu, S. S.
Curr. Opin. Biotech. 12, 340-7. (2001)
Year 2000 and Earlier
Phage display in pharmaceutical biotechnology.
Sidhu, S. S.
Curr. Opin. Biotechnol. 11, 610-6. (2000)
Phage display for selection of novel binding peptides.
Sidhu, S. S., Lowman, H. B., Cunningham, B. C. & Wells, J. A.
Methods Enzymol. 328, 333-63. (2000)
Efficient phage display of polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein.
Fuh, G. & Sidhu, S. S.
FEBS Lett. 480, 231-4. (2000)
Rapid mapping of protein functional epitopes by combinatorial alanine scanning.
Weiss, G. A., Watanabe, C., Zhong, A., Goddard A. & Sidhu, S. S.
Proc. Natl. Acad. Sci. U.S.A. 97, 8950-4. (2000)
Analysis of PDZ domain-ligand interactions using carboxyl-terminal phage display.
Fuh, G., Pisabarro, M. T., Li, Y., Quan, C., Lasky, L. A. & Sidhu, S. S.
J. Biol. Chem. 275, 21486-91. (2000)
Design and evolution of artificial M13 coat proteins.
Weiss, G. A. & Sidhu, S. S.
J. Mol. Biol. 300, 213-9. (2000)
Mutational analysis of the major coat protein of M13 identifies residues that control protein display.
Weiss, G. A., Wells, J. A. & Sidhu, S. S.
Protein Sci. 9, 647-54. (2000)
High copy display of large proteins on phage for functional selections.
Sidhu, S. S., Weiss, G. A. & Wells, J. A.
J. Mol. Biol. 296, 487-95. (2000)
Streptomyces griseus protease B - Secretion correlates with the length of the propeptide.
Baardsnes, J., Sidhu, S., Macleod, A., Elliott, J., Morden, D. Watson, J. & Borgford, T.
J. Bacteriol. 180, 3241-4. (1998)
Selection of Streptomyces griseus protease B mutants with desired alterations in primary specificity using a library screening strategy.
Sidhu, S. S. & Borgford T. J.
J. Mol. Biol. 257, 233-45. (1996)
Protease evolution in Streptomyces griseus: Discovery of a novel dimeric enzyme.
Sidhu, S. S., Kalmar, G. B., Willis, L. G. & Borgford, T. J.
J. Biol. Chem. 270, 7594-600. (1995)
Streptomyces griseus protease C: A novel enzyme of the chymotrypsin superfamily.
Sidhu, S. S., Kalmar, G. B., Willis, L. G. & Borgford, T. J.
J. Biol. Chem. 269, 20167-71. (1994)
Characterization of the gene encoding the glutamic-acid-specific protease of Streptomyces griseus.
Sidhu, S. S., Kalmar, G. B. & Borgford, T. J.
Biochem. Cell Biol. 71, 454-61. (1993)