[MacLennan Lab] [UofT]
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Sessions
+ Molecular Structure
+ Genetic diseases
+ RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
+ RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
+ Elementary Events
+ Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

 

The Gordon Research Conference
on
 Muscle: Excitation-Contraction Coupling

Colby-Sawyer College, New London, New Hampshire

June 11-16, 2000

Organizers

Dr. David H. MacLennan
e-mail: david.maclennan@utoronto.ca
home page: http://www.utoronto.ca/maclennan/
Dr. Martin F. Schneider
e-mail: mschneid@umaryland.edu
home page: http://som1.umaryland.edu/muscle/home.html

Platform Sessions:

 
Sessions
> Molecular Structure
+ Genetic diseases
+ RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
+ RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
+ Elementary Events
+ Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

Sunday, June 11, 2000

Evening Session

I. MOLECULAR STRUCTURE OF E-C COUPLING COMPONENTS

Leaders:
David Stokes mailto:stokes@saturn.med.nyu.edu
Terry Wagenknecht mailto:terry@wadsworth.org

Time Speaker Topic

19:45-20:00 Terry Wagenknecht Introduction to the structural basis of e-c coupling.
20:00-20:30 Eduardo Perozo Molecular basis of proton-dependent activation gating in KcsA.
20:30-21:00 Angela Dulhunty Structure of an RyR binding domain on the skeletal DHPR II-III loop: consequences for activation or inhibition of RyR.
21:00-21:30 Wah Chiu Electron cryomicroscopy of skeletal muscle Ca2+ release channels in different states.
21:30-22:00 ChulHee Kang Structure of calsequestrin: implications for calcium regulation.
22:00-22:30 David Stokes Structure and dynamics of the Ca2+ ATPase by electron microscopy.


Sessions
+ Molecular Structure
> Genetic diseases
+ RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
+ RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
+ Elementary Events
+ Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

Monday, June 12, 2000

Morning Session

II. GENETIC DISEASE RESULTING FROM DEFECTS IN E-C COUPLING PROTEINS

Leaders:
Frank Lehmann-Horn mailto:frank.lehmann-horn@medizin.uni-ulm.de
Charles Louis mailto:louis003@maroon.tc.umn.edu

Time Speaker Topic

9:00-9:15 Frank Lehmann-Horn News and views in channelopathies.
9:15-9:45 Karin Jurkat-Rott A second gene for hypokalemic periodic paralysis.
9:45-10:15 Tommie McCarthy Malignant hyperthermia susceptibility and central core disease: what causes the difference.
10:15-10:45 Coffee break and group picture.
10:45-11:15 Jacques Veerkamp Alterations in native and cultured muscle of Brody disease patients.
11:15-11:30 David MacLennan Molecular genetics of Brody disease.
11:30-12:00 Steve Goldstein Channels involved in long QT syndrome and ventricular fibrillation.
12:00-12:30 Poster highlights.

Sessions
+ Molecular Structure
+ Genetic diseases
> RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
+ RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
+ Elementary Events
+ Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

Evening Session

III. STRUCTURE-FUNCTION ANALYSIS OF RYANODINE RECEPTORS

Leaders:
Jianje Ma mailto:jxm63@po.cwru.edu
Wayne Chen mailto:swchen@acs.ucalgary.ca

Time Speaker Topic

19:00-19:15 Jianjie Ma Limits and difficulties in the functional expression of ryanodine receptors in heterologous cells
19:15-19:45 GuoGuang Du The use of chimera formation and site-directed mutagenesis to analyze structure/function relationships in the ryanodine receptor.
19:45-20:15 Manjunatha Bhat Membrane topology and localization of ryanodine receptor calcium release channel.
20:15-20:45 Gerhard Meissner Probing the ion conducting pore of the Ca2+ release channel.
20:45-21:15 Gregory Mignery InsP3 receptor structure and function: "Pore Progress".
21:15-21:45 Wayne Chen Isoform-specific formation of heteromeric ryanodine receptor Ca2+ release channels.
21:45-22:15 Poster highlights.


Sessions
+ Molecular Structure
+ Genetic diseases
+ RyR Structure -Function analysis
> RyR / a1-DHPR Interaction
+ RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
+ Elementary Events
+ Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

Tuesday, June 13, 2000

Morning Session

IV. INTERACTIONS BETWEEN RYANODINE RECEPTORS AND a1-DIHYDROPYRIDINE RECEPTORS

Leaders:
Paul Allen mailto:allen@zeus.bwh.harvard.edu
Clara Franzini-Armstrong mailto:armstroc@mail.med.upenn.edu

Time Speaker Topic

09:00-09:30 Clara Franzini-Armstrong DHPR-RyR: spatial relationships.
09:30-10:00 Kurt Beam Bi-directional signalling between DHPRs and RyRs.
10:00-10:30 Paul Allen EC-Coupling, orthograde and retrograde signalling through RyR1.
10:30-11:00 coffee break
11:00-11:30 Noriaki Ikemoto Peptide probe study of the signal transmission mechanism.
11:30-12:00 Hector Valdivia Activation of ryanodine receptors by Imperatoxin A and a peptide segment of the II-III loop of the dihydropyridine receptor.
12:00-12:30 Bernhard Flucher Molecular domains involved in targeting and modulation of the skeletal muscle DHP-Receptor.

Sessions
+ Molecular Structure
> Genetic diseases
+ RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
> RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
+ Elementary Events
+ Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

Evening Session

V. MODULATION OF RYANODINE RECEPTOR FUNCTION BY ACCESSORY PROTEINS AND BY PROTEIN MODIFICATION

Leaders:
Susan Hamilton mailto:susanh@bcm.tmc.edu
Andy Marks mailto:arm42@columbia.edu

Time Speaker Topic

19:30-20:00 Andy Marks The ryanodine receptor as a macromolecular complex.
20:00-20:30 Sidney Fleischer Ryanodine receptors and FK-Binding Proteins: Isoforms, association and function.
20:30-21:00 Susan Hamilton Functional domains of RyR1.
21:00-21:30 Charles Louis Isoform-specific regulation of ryanodine receptors by calmodulin and dantrolene.
21:30-22:00 Francesco Zorzato Molecular and functional characterization of junctional sarcoplasmic reticulm proteins.
22:00-22:30 Meredith Bond A-kinase anchoring protein targeting of PKA to junctional sarcoplasmic reticulum in cardiac muscle.


Sessions
+ Molecular Structure
+ Genetic diseases
+ RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
+ RyR modulation: Protein
> RyR modulation: Gradients & Drugs
+ Elementary Events
+ Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

Wednesday, June 14, 2000

Morning Session

VI. MODULATION OF RYANODINE RECEPTOR FUNCTION BY TRANSMEMBRANE GRADIENTS AND PHARMACOLOGICAL AGENTS

Leaders:
Isaac Pessah mailto:inpessah@ucdavis.edu
Alan Williams mailto:a.j.williams@ic.ac.uk

Time Speaker Topic

09:00-09:30 Isaac Pessah Variations in sensitivity of Ryr isoforms to physiologic and pharmacologic modulators.
09:30-10:00 Graham Lamb Effects of SR Ca2+loading level and cytoplasmic factors on Ca2+ release by depolarization or caffeine in skinned fibers.
10:00-10:30 Sandor Gyorke Ca2+ regulation of ryanodine receptors.
10:30-11:00 coffee break
11:00-11:30 Poster highlights
11:30-12:00 Cecilia Hidalgo Effects of SH oxidation on the regulation of ryanodine receptor function by Ca2+ and Mg2+.
12:00-12:30 Alan Williams The interaction of novel ryanoids with the single RyR channels.

Sessions
+ Molecular Structure
+ Genetic diseases
+ RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
+ RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
> Elementary Events
+ Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

Evening Session

VII. ELEMENTARY EVENTS OF Ca2+ RELEASE

Leaders:
Eduardo Rios mailto:erios@rush.edu
Gil Weir mailto:gwier001@umaryland.edu

Time Speaker Topic

19:30-19:45 Eduardo Rios Unanswered questions about Ca2+ sparks
19:45-20:15 Ernst Niggli Cellular and subcellular refractoriness of Ca2+-release in cardiac muscle.
20:15-20:45 Heping Cheng Coupling of single L-type channels to Ca2+ sparks in heart.
20:45-21:15 Ian Parker Puffs, blips, sparks and quarks: comparative anatomy of elementary Ca2+ events.
21:15-21:45 Chris Ward Modulation of local Ca2+ release events in skeletal muscle.
21:45-22:15 Adom Gonzalez Events induced by Ca release agonists in skeletal muscle. Insights from video-rate scanning microscopy.
22:15-22:30 Poster highlights
Michael Klein How many Ca2+ sparks can be released in a single sarcomere?


Sessions
+ Molecular Structure
+ Genetic diseases
+ RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
+ RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
+ Elementary Events
> Ca2+ Release & Reuptake
+ RyR3, IP3R and other Proteins

o Gordon Research Conferences

Thursday, June 15, 2000

Morning Session

VIII. PHYSIOLOGICAL Ca2+ RELEASE AND REUPTAKE

Leaders:
Knox Chandler mailto:knox.chandler@yale.edu
Donald Bers mailto:dbers@luc.edu

Time Speaker Topic

09:00-09:45 Don Bers Integrated Ca2+ management in heart cells.
Poster highlights
Godfrey Smith Effects of reduced [ATP] on spontaneous Ca2+ release in permeabilized single cardiac cells
09:45-10:15 Mike Stern Local control theory in cardiac muscle.
10:15-10:45 coffee break
10:45-11:15 Steve Baylor Ca2+ release and spread within the sarcomere of intact skeletal muscle fibers.
11:15-12:00 Natalia Shirokova Control of Ca2+ release in mammalian skeletal muscle.
Poster highlights
Carlo Caputo Potentiation of Ca2+ release affects its Ca2+ dependent inactivation.
12:00-12:30 David Allen Ca2+ release, Ca2+ stores and muscle fatigue.

Sessions
+ Molecular Structure
+ Genetic diseases
+ RyR Structure -Function analysis
+ RyR / a1-DHPR Interaction
+ RyR modulation: Protein
+ RyR modulation: Gradients & Drugs
+ Elementary Events
+ Ca2+ Release & Reuptake
> RyR3, IP3R and other Proteins

o Gordon Research Conferences

Evening Session

IX. RyR3, IP3R AND OTHER E/C COUPLING PROTEINS IN STRIATED MUSCLE

Leaders:
Barbara Block mailto:bblock@leland.stanford.edu
Yasuo Ogawa mailto:ysogawa@med.juntendo.ac.jp

Time Speaker Topic

19:30-20:00 Yasuo Ogawa Different contributions between alpha-RyR and beta-RyR to CICR activity in sarcoplasmic reticulum vesicles from frog skeletal muscles.
20:00-20:15 Barbara Block Fiber type specific ryanodine receptors in fish.
20:15-20:45 Vincenzo Sorrentino Functional properties and mechanisms of subcellular localization of RyR3 channels.
20:45-21:15 Hiroshi Takeshima Mitsugumins - new protein components of the triad junction.
21:15-21:45 Masamitsu Iino Structure-function relationships in the IP3 receptor.
21:45-22:15 Enrique Jaimovich Function and subcellular localization of IP3R in muscle cells.

Revised
1999Dec27