The Gordon Research Conference
on
 Muscle: Excitation-Contraction Coupling

Colby-Sawyer College, New London, New Hampshire

June 11-16, 2000

Platform Sessions:

Sunday, June 11, 2000

Evening Session

I. MOLECULAR STRUCTURE OF E-C COUPLING COMPONENTS

Leaders:
David Stokes mailto:stokes@saturn.med.nyu.edu
Terry Wagenknecht mailto:terry@wadsworth.org

Time	Speaker		Topic
19:45-20:00	Terry Wagenknecht		Introduction to the structural basis of e-c coupling.
20:00-20:30	Eduardo Perozo		Molecular basis of proton-dependent activation gating in KcsA.
20:30-21:00	Angela Dulhunty		Structure of an RyR binding domain on the skeletal DHPR II-III loop: consequences for activation or inhibition of RyR.
21:00-21:30	Wah Chiu		Electron cryomicroscopy of skeletal muscle Ca2+ release channels in different states.
21:30-22:00	ChulHee Kang		Structure of calsequestrin: implications for calcium regulation.
22:00-22:30	David Stokes		Structure and dynamics of the Ca2+ ATPase by electron microscopy.

Monday, June 12, 2000

Morning Session

II. GENETIC DISEASE RESULTING FROM DEFECTS IN E-C COUPLING PROTEINS

Leaders:
Frank Lehmann-Horn mailto:frank.lehmann-horn@medizin.uni-ulm.de
Charles Louis mailto:louis003@maroon.tc.umn.edu

Time	Speaker		Topic
9:00-9:15	Frank Lehmann-Horn		News and views in channelopathies.
9:15-9:45	Karin Jurkat-Rott		A second gene for hypokalemic periodic paralysis.
9:45-10:15	Tommie McCarthy		Malignant hyperthermia susceptibility and central core disease: what causes the difference.
10:15-10:45	Coffee break and group picture.
10:45-11:15	Jacques Veerkamp		Alterations in native and cultured muscle of Brody disease patients.
11:15-11:30	David MacLennan		Molecular genetics of Brody disease.
11:30-12:00	Steve Goldstein		Channels involved in long QT syndrome and ventricular fibrillation.
12:00-12:30	Poster highlights.

Evening Session

III. STRUCTURE-FUNCTION ANALYSIS OF RYANODINE RECEPTORS

Leaders:
Jianje Ma mailto:jxm63@po.cwru.edu
Wayne Chen mailto:swchen@acs.ucalgary.ca

Time	Speaker		Topic
19:00-19:15	Jianjie Ma		Limits and difficulties in the functional expression of ryanodine receptors in heterologous cells
19:15-19:45	GuoGuang Du		The use of chimera formation and site-directed mutagenesis to analyze structure/function relationships in the ryanodine receptor.
19:45-20:15	Manjunatha Bhat		Membrane topology and localization of ryanodine receptor calcium release channel.
20:15-20:45	Gerhard Meissner		Probing the ion conducting pore of the Ca2+ release channel.
20:45-21:15	Gregory Mignery		InsP3 receptor structure and function: "Pore Progress".
21:15-21:45	Wayne Chen		Isoform-specific formation of heteromeric ryanodine receptor Ca2+ release channels.
21:45-22:15	Poster highlights.

Tuesday, June 13, 2000

Morning Session

IV. INTERACTIONS BETWEEN RYANODINE RECEPTORS AND a1-DIHYDROPYRIDINE RECEPTORS

Leaders:
Paul Allen mailto:allen@zeus.bwh.harvard.edu
Clara Franzini-Armstrong mailto:armstroc@mail.med.upenn.edu

Time	Speaker		Topic
09:00-09:30	Clara Franzini-Armstrong		DHPR-RyR: spatial relationships.
09:30-10:00	Kurt Beam		Bi-directional signalling between DHPRs and RyRs.
10:00-10:30	Paul Allen		EC-Coupling, orthograde and retrograde signalling through RyR1.
10:30-11:00	coffee break
11:00-11:30	Noriaki Ikemoto		Peptide probe study of the signal transmission mechanism.
11:30-12:00	Hector Valdivia		Activation of ryanodine receptors by Imperatoxin A and a peptide segment of the II-III loop of the dihydropyridine receptor.
12:00-12:30	Bernhard Flucher		Molecular domains involved in targeting and modulation of the skeletal muscle DHP-Receptor.

Evening Session

V. MODULATION OF RYANODINE RECEPTOR FUNCTION BY ACCESSORY PROTEINS AND BY PROTEIN MODIFICATION

Leaders:
Susan Hamilton mailto:susanh@bcm.tmc.edu
Andy Marks mailto:arm42@columbia.edu

Time	Speaker		Topic
19:30-20:00	Andy Marks		The ryanodine receptor as a macromolecular complex.
20:00-20:30	Sidney Fleischer		Ryanodine receptors and FK-Binding Proteins: Isoforms, association and function.
20:30-21:00	Susan Hamilton		Functional domains of RyR1.
21:00-21:30	Charles Louis		Isoform-specific regulation of ryanodine receptors by calmodulin and dantrolene.
21:30-22:00	Francesco Zorzato		Molecular and functional characterization of junctional sarcoplasmic reticulm proteins.
22:00-22:30	Meredith Bond		A-kinase anchoring protein targeting of PKA to junctional sarcoplasmic reticulum in cardiac muscle.

Wednesday, June 14, 2000

Morning Session

VI. MODULATION OF RYANODINE RECEPTOR FUNCTION BY TRANSMEMBRANE GRADIENTS AND PHARMACOLOGICAL AGENTS

Leaders:
Isaac Pessah mailto:inpessah@ucdavis.edu
Alan Williams mailto:a.j.williams@ic.ac.uk

Time	Speaker		Topic
09:00-09:30	Isaac Pessah		Variations in sensitivity of Ryr isoforms to physiologic and pharmacologic modulators.
09:30-10:00	Graham Lamb		Effects of SR Ca2+loading level and cytoplasmic factors on Ca2+ release by depolarization or caffeine in skinned fibers.
10:00-10:30	Sandor Gyorke		Ca2+ regulation of ryanodine receptors.
10:30-11:00	coffee break
11:00-11:30	Poster highlights
11:30-12:00	Cecilia Hidalgo		Effects of SH oxidation on the regulation of ryanodine receptor function by Ca2+ and Mg2+.
12:00-12:30	Alan Williams		The interaction of novel ryanoids with the single RyR channels.

Evening Session

VII. ELEMENTARY EVENTS OF Ca²⁺ RELEASE

Leaders:
Eduardo Rios mailto:erios@rush.edu
Gil Weir mailto:gwier001@umaryland.edu

Time	Speaker		Topic
19:30-19:45	Eduardo Rios		Unanswered questions about Ca2+ sparks
19:45-20:15	Ernst Niggli		Cellular and subcellular refractoriness of Ca2+-release in cardiac muscle.
20:15-20:45	Heping Cheng		Coupling of single L-type channels to Ca2+ sparks in heart.
20:45-21:15	Ian Parker		Puffs, blips, sparks and quarks: comparative anatomy of elementary Ca2+ events.
21:15-21:45	Chris Ward		Modulation of local Ca2+ release events in skeletal muscle.
21:45-22:15	Adom Gonzalez		Events induced by Ca release agonists in skeletal muscle. Insights from video-rate scanning microscopy.
22:15-22:30	Poster highlights
	Michael Klein		How many Ca2+ sparks can be released in a single sarcomere?

Thursday, June 15, 2000

Morning Session

VIII. PHYSIOLOGICAL Ca²⁺ RELEASE AND REUPTAKE

Leaders:
Knox Chandler mailto:knox.chandler@yale.edu
Donald Bers mailto:dbers@luc.edu

Time	Speaker		Topic
09:00-09:45	Don Bers		Integrated Ca2+ management in heart cells.
	Poster highlights
	Godfrey Smith		Effects of reduced [ATP] on spontaneous Ca2+ release in permeabilized single cardiac cells
09:45-10:15	Mike Stern		Local control theory in cardiac muscle.
10:15-10:45	coffee break
10:45-11:15	Steve Baylor		Ca2+ release and spread within the sarcomere of intact skeletal muscle fibers.
11:15-12:00	Natalia Shirokova		Control of Ca2+ release in mammalian skeletal muscle.
	Poster highlights
	Carlo Caputo		Potentiation of Ca2+ release affects its Ca2+ dependent inactivation.
12:00-12:30	David Allen		Ca2+ release, Ca2+ stores and muscle fatigue.

Evening Session

IX. RyR3, IP₃R AND OTHER E/C COUPLING PROTEINS IN STRIATED MUSCLE

Leaders:
Barbara Block mailto:bblock@leland.stanford.edu
Yasuo Ogawa mailto:ysogawa@med.juntendo.ac.jp

Time	Speaker		Topic
19:30-20:00	Yasuo Ogawa		Different contributions between alpha-RyR and beta-RyR to CICR activity in sarcoplasmic reticulum vesicles from frog skeletal muscles.
20:00-20:15	Barbara Block		Fiber type specific ryanodine receptors in fish.
20:15-20:45	Vincenzo Sorrentino		Functional properties and mechanisms of subcellular localization of RyR3 channels.
20:45-21:15	Hiroshi Takeshima		Mitsugumins - new protein components of the triad junction.
21:15-21:45	Masamitsu Iino		Structure-function relationships in the IP3 receptor.
21:45-22:15	Enrique Jaimovich		Function and subcellular localization of IP3R in muscle cells.