Revealing global regulatory features of mammalian alternative splicing using a quantitative microarray platform

Qun Pan ^1,2, Ofer Shai ^1,3, Christine Misquitta ², Wen Zhang ^2,4, Naveed Mohammed ², Tomas Babak ^2,4, Henry Siu ^2,4, Timothy R. Hughes ^2,4,5, Quaid D. Morris ^2,3,6, Brendan Frey ^3,6, and Benjamin J. Blencowe ^2,4-7

• Table S1. List of 3,126 microarray-monitored AS events, GenASAP %ASex values and rankings [3 MB]
• Table S2. Supporting cDNA/EST data for the 3,126 microarray-monitored AS events [5 MB]
• Table S3. Microarray-monitored AS events that result in domain modification
• Table S4. Gene Ontology annotations for microarray-monitored AS genes

• Figure S1. Reproducibility of GenASAP measurements for 54 duplicate AS events.
• Figure S2. RT-PCR validation of alternative exon splicing levels predicted using microarray data from ten mouse tissues.* [download powerpoint]
• Figure S3. Inferring the consequences of tissue-specific AS on protein structure and function using microarray data.* [download powerpoint]

• Experimental Procedures

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¹ Co-first authors
⁶ Co-senior authors

² Banting and Best Department of Medical Research,
³ Computer Science and Engineering,
⁴ Department of Molecular and Medical Genetics,
⁵ Program in Proteomics and Bioinformatics,
University of Toronto

⁷ To whom correspondence should be addressed. Email: b.blencowe@utoronto.ca