Dr. Morris Frank Manolson	Dr. Morris Frank Manolson Associate Dean, Graduate and Postgraduate Studies Associate Professor, Faculty of Dentistry Assistant Professor, Department of Biochemistry, University of Toronto Faculty of Dentistry 124 Edward Street Toronto, Ontario, Canada M5G 1G6 Office: 4416-979-4900 ext. 1-4392 Lab: 416-979-4900 ext. 1-4574 Fax: 416-979-4936 m.manolson@utoronto.ca Bonelab Profile - Department of Biochemistry
Current academic / hospital appointments
Associate Professor, Faculty of Dentistry Cross-appointed to the Dept. of Biochemistry, Faculty of Medicine
Education
B.S. with Distinction, Cell and Molecular Biology, McGill University, 1981 German Academic Foreign Exchange Student, Free University of Berlin, 1981 - 1983 Ph.D., Dean's Honour List, Cell and Molecular Biology, McGill University, 1983 - 1988 (Thesis Advisor: Prof. Ronald J. Poole) Postdoctoral Fellow, Carnegie Mellon University, 1988 – 1992 (Postdoctoral Advisor: Prof. Elizabeth W. Jones)
Courses
Undergraduate: Biochem. for 1st year Medical students, MEDS1 seminars, 2003/07; Biological basis of oral health and disease 121Y1, 2003-2009; Molecular Aspect of Ion Transport BCH2021H, Spring 2003; Special Topics JLM349 (membrane transport), spring 2002; Biology of Connective Tissues, DEN1080Y, 2001, 2004/06/07/08/10 Graduate / Postgraduate: Course coordinator for “Seminars in Oral Health”, DEN1001Y, 2008-present; Biochemistry BCH 2021, Graduate level, Fall, 2000; Cell Surface Biochemistry B422S, Undergrad./Grad level, 1993-1997; Molecular Biology of Membrane, Transporters, Biochemistry 20215 Undergraduate/Graduate level, 1996
Recent publications
Ochotny, O., Flenniken, A.M., Owen, C., Voronov, I., Zirngibl, R.A., Osborne, L.R., Henderson, J.E., Adamson, S.L., Rossant, J., Manolson, M.F., Aubin, J. The V ATPase a3 subunit mutation R740S is dominant negative and results in osteopetrosis in mice. Accepted by Journal of Bone and Mineral Research manuscript number J1008569 in January 2011. 2. Durand, M., Boire, G., Komarova, S.V., Dixon, S.J., Sims, S.M., Harrison, R.E., Nabavi, N., Manolson, M.F., Mizianty, M., Kurgan L., and de Brum-Fernandes, A.J. The increased ex vivo osteoclastogenesis in patients with rheumatiod arthritis is due to increased number of precursors and decreased apoptosis-the in vitro osteoclast differentiation in arthrtitis (IODA) study. Bone. 2011 Mar 1;48(3):588-96. Kartner, N., Yao, Y., K. Li, Gazelle J. Crasto, A. Datti and M.F. Manolson. Inhibition of Osteoclast V-ATPase-mediated Acid Secretion by Targeting the a3-B2 Subunit Interaction. J Biol Chem. 2010 Nov 26;285(48):37476-90. Gramoun, A., Azizi, N., Sodek, J., Heersche, J.N.M., Nakchbandi, I., and Manolson, M.F. Fibronectin inhibits osteoclastogenesis while enhancing osteoclast activity via nitric oxide and interleukin-1β-mediated signaling pathways. J Cell Biochem. 2010 Nov 1;111(4):1020-34 Gramoun, A., Goto, T., Nosrdstrom, T., Rotstein, O., Grinstein, S., Heersche, J.N., M.F. Manolson. Bone Matrix Proteins and Extracellular Acidification; Potential Co-regulators of Osteoclast Morphology. J Cell Biochem. 2010 Oct 1;111(2):350-61.
Students
2003 - Noelle Ochotny (co-supervised with Dr. Aubin): Effect of V-ATPase a3 R740S mutation on osteoclastogenesis 2008 - Natoosha Azizi: Defining interactions between a3/B2/d2 VATPase subunits 2009 - Gazelle Crasto: Defining mechanisms of human a2 and a4 mutations 2009 - Ajay Bhargava: Non classical roles of V-ATPases within osteoclasts
Current research grants
Funds Currently Held 1. Source of Funding: Canadian Institutes of Health Research   Program: Operating Grant   Title: Characterizing mutations in the a3 V-ATPase subunit in osteoclasts: A key player in bone loss due to inflammatory arthritis (CIHR application #230683)   Principal investigator: Dr. M.F. Manolson   Co-investigator Jane Aubin   Amount/Year: $122,418   Support Period 01/2011-03/2016   Renewable: Yes     2. Source of Funding: CIHR IMHA   Program: New Emerging Team   Title: Role of osteoclastogenesis and osteoclast activation in joint destruction in degenerative and inflammatory joint diseases   Principal investigator: Dr. M.F. Manolson   Role Principal Investigator   Amount/Year: $300,000   Support Period 2007 - 2011   Renewable: Yes     3. Source of Funding: Canadian Institutes of Health Research   Program: Operating Grant   Title: Unravelling the pathogenesis of the Autophagic Vacuolar Myopathies (CIHR grant FRN MOP-64041)   Principal investigator: Berge Minassian   Role: Co-applicant   Amount/Year: $91,623   Support Period Apr 1, 2007 - Mar 31, 2012   Renewable: Yes