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Membrane proteomics | ||||||||||||||||
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Membrane proteins comprise the largest set of proteins to resist high-throughput structural genomics efforts. One of the major impediments to the analysis of membrane proteins is the lack of generic and effective expression systems. The aims of the membrane protein platform are to develop the methodologies to perform high-throughput cloning, expression, purification and crystallization of membrane proteins. To date, we have purified over 30 targets to homogeneity. This represents ~10% of the total number of genes cloned (compared to an average of 40% for similar efforts with soluble proteins). The proteins we have purified include several active prokaryotic and eukaryotic rhomboid proteases. We were also involved in the structure determination of the CorA Mg2+ Transporter. See Target summary for our E.coli/T.Maritima protein project |  |
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Site last updated April 2006 | |||